rabbit polyclonal anti-h4k5ac  (PTM Biolabs)

Journal: Planta

Article Title: Epigenetic modifications evidenced by isolation of proteins on nascent DNA and immunofluorescence in hydroxyurea-treated root meristem cells of Vicia faba

doi: 10.1007/s00425-023-04249-2

Figure Lengend Snippet: Immunoblot analysis and densitometry graphs showing post-translational histone modifications. a Input and EdU-associated proteins (iPOND) were analysed by Western blot using antibodies against H3K56Ac, H4K5Ac, H3T45Ph, and H3. b Densitometry plots showing the intensity of the bands (for the SDS-PAGE gel). c Densitometric plots of iPOND/Western blot bands for control and HU showing the ratio of histone modifications H3K56Ac, H4K5Ac, H3T45Ph to total histone H3. d DNA fragmentation detected with agarose gel electrophoresis of DNA extracted V. faba root meristem cells: lane 1—mass marker, lane 2—control, and lane 3—HU treatment for 2 h. Statistically significant changes (± SD) in the intensity of the bands (for the SDS-PAGE gel) (± SD) were assessed with Student’s t–test. The lack of significance is indicated by “n.s.” (not significant)

Article Snippet: Slides were then incubated with: rabbit polyclonal anti-histone H4K5Ac antibodies (Sigma-Aldrich, dilution of 1:200); rabbit monoclonal anti-histone H3K56Ac antibodies (Abcam, dilution of 1:200); rabbit polyclonal anti-histone H3T45Ph antibodies (Abcam, dilution of 1:100).

Techniques: Western Blot, SDS Page, Agarose Gel Electrophoresis, Marker

Journal: Planta

Article Title: Epigenetic modifications evidenced by isolation of proteins on nascent DNA and immunofluorescence in hydroxyurea-treated root meristem cells of Vicia faba

doi: 10.1007/s00425-023-04249-2

Figure Lengend Snippet: Intranuclear distribution of H4K5Ac immunofluorescence. a , b Cell nuclei with immunostained euchromatin areas: a control, b HU-treated cells and corresponding images of cell nuclei stained with propidium iodide ( a ’) and DAPI ( b ’). c , d Cell nuclei with immunostained heterochromatin areas: c control, d HU-treated cells and corresponding images of cell nuclei stained with propidium iodide ( c ’) and DAPI ( d ’). e – f Cell nuclei with nucleolar staining: e control, f HU-treated cells and corresponding images of cell nuclei stained with propidium iodide ( e ’) and DAPI ( f ’). Scale bar = 10 μm

Article Snippet: Slides were then incubated with: rabbit polyclonal anti-histone H4K5Ac antibodies (Sigma-Aldrich, dilution of 1:200); rabbit monoclonal anti-histone H3K56Ac antibodies (Abcam, dilution of 1:200); rabbit polyclonal anti-histone H3T45Ph antibodies (Abcam, dilution of 1:100).

Techniques: Immunofluorescence, Staining

Journal: Planta

Article Title: Epigenetic modifications evidenced by isolation of proteins on nascent DNA and immunofluorescence in hydroxyurea-treated root meristem cells of Vicia faba

doi: 10.1007/s00425-023-04249-2

Figure Lengend Snippet: a–c Frequencies (% ± SD) of nuclei with H4K5Ac immunolabeling localized in: a euchromatic, b heterochromatic, c and nucleolar regions, discriminated with respect to successive stages of the cell cycle in root meristems. Statistically significant changes in the intensity of H4K5Ac fluorescence (± SD) were assessed with Student’s t -test

Article Snippet: Slides were then incubated with: rabbit polyclonal anti-histone H4K5Ac antibodies (Sigma-Aldrich, dilution of 1:200); rabbit monoclonal anti-histone H3K56Ac antibodies (Abcam, dilution of 1:200); rabbit polyclonal anti-histone H3T45Ph antibodies (Abcam, dilution of 1:100).

Techniques: Immunolabeling, Fluorescence

Journal: Planta

Article Title: Epigenetic modifications evidenced by isolation of proteins on nascent DNA and immunofluorescence in hydroxyurea-treated root meristem cells of Vicia faba

doi: 10.1007/s00425-023-04249-2

Figure Lengend Snippet: a–c H4K5Ac immunofluorescence labeling of the chromosomal areas corresponding to the nucleolus-organizing regions (NORs) in the control root meristems during: prometaphase ( a ), anaphase ( b ), and in the HU-treated cells during metaphase ( c ). The corresponding images of cell nuclei stained with propidium iodide ( a ’, b ’) and DAPI ( c ’). d , e AgNOR staining of the control chromosomes: NOR-carrying isolated metaphase chromosomes. Arrows show NORs regions. Scale bar = 10 μm

Article Snippet: Slides were then incubated with: rabbit polyclonal anti-histone H4K5Ac antibodies (Sigma-Aldrich, dilution of 1:200); rabbit monoclonal anti-histone H3K56Ac antibodies (Abcam, dilution of 1:200); rabbit polyclonal anti-histone H3T45Ph antibodies (Abcam, dilution of 1:100).

Techniques: Immunofluorescence, Labeling, Staining, Isolation